The Laboratory of Excellence NetRNA gathers 11 teams from 3 CNRS units located at IBMC (Institut de Biologie Moléculaire et Cellulaire/Institute of Molecular and Cellular Biology) and IBMP (Institut de Biologie Moléculaire des Plantes/ Institute of Plant Molecular Biology). The teams investigated the manifold functions of the non-coding genome across kingdoms, leading to a unique “integrated RNA research institute” in France to study regulatory RNAs and their machineries in infectious diseases offering advanced interdisciplinary and innovative training. The project of NetRNA has evolved to generate an integrated view of the RNA-based strategies developed by pathogens and their hosts during infection and on the insect strategies to resist viral infection. To make full use of our diverse biological models and their phylogenetic comparison, the omics, biochemical, and structural data we collect will be integrated using network modeling approaches. As innovative methodologies, we will use: (i) microfluidics for single-cell studies; (ii) mass spectrometry (MS) and direct RNA sequencing for RNA modifications; (iii) sophisticated genetic and biochemical approaches to identify key signaling pathways, interconnected pathways, and cellular RNA machineries.
Task 1: Molecular machineries of regulatory RNAs (HTS, MS)
- Understanding of regulatory RNAs, their targets, and the multi-protein effector complexes involved in their functionality and regulation
- Elucidate the regulatory mechanisms at the molecular level and rules that dictate specific recognition between RNAs and their targets
Task 2: Regulatory RNAs, biotic and abiotic stresses (HTS, imaging)
- Profiling regulatory RNAs under stress conditions
- Impact of biotic and abiotic stresses on molecular machineries
- Localization of regulatory RNAs and their molecular machineries
Task 3: Regulatory RNAs: towards system biology (HTS, microfluidics, bioinformatics)
- Establishment of networks
- Dynamics of the networks: single cell studies
