Joao Trinidade Marquès, Group Leader at UPR 9022 – M3i CNRS
![MARQUES Joao](https://labex-netrna.cnrs.fr/wp-content/uploads/2021/05/MARQUES_Joao.jpg)
Contact
Joao Trinidade Marquès
Phone
E-Mail
Website
UPR 9022 – M3I CNRS
Institut de Biologie Moléculaire et Cellulaire du CNRS
2 allée Konrad Roentgen
67084 Strasbourg Cedex
France
Research topics
- The biology of antiviral RNA silencing in Aedes mosquitoes
- Cellular and molecular mechanisms that contribute to the control arbovirus infections in Aedes mosquitoes
- Characterization of the natural virome of Aedes mosquitoes and its impact on vector competence
Role in NetRNA
The group will study cellular and molecular aspects of host defence mechanisms, such as RNA silencing, that play a role in the control of arbovirus infections in Aedes mosquitoes. In addition, the group will use RNA sequencing approaches to identify novel biological pathways that are involved in natural resistance to arboviruses observed in wild populations of Aedes mosquitoes. Finally, the group will further develop metagenomic strategies based on the production of virus-derived small RNAs within infected hosts to identify and analyze the virome of wild Aedes mosquitoes worldwide.
Working Group
Publications
2021
Almeida, J P De; Aguiar, E R; Armache, J N; Olmo, R P; Marques, J T
The virome of vector mosquitoes Article de journal
Dans: Curr Opin Virol, vol. 49, p. 7-12, 2021, ISBN: 33991759, (1879-6265 (Electronic) 1879-6257 (Linking) Journal Article Review).
@article{nokey,
title = {The virome of vector mosquitoes},
author = {J P De Almeida and E R Aguiar and J N Armache and R P Olmo and J T Marques},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=33991759},
doi = {10.1016/j.coviro.2021.04.002},
isbn = {33991759},
year = {2021},
date = {2021-01-01},
journal = {Curr Opin Virol},
volume = {49},
pages = {7-12},
abstract = {Mosquitoes are the major vectors for arthropod-borne viruses (arboviruses) of medical importance. Aedes aegypti and A. albopictus are the most prolific and widespread mosquito vectors being responsible for global transmission of dengue, Zika and Chikungunya viruses. Characterizing the collection of viruses circulating in mosquitoes, the virome, has long been of special interest. In addition to arboviruses, mosquitoes carry insect-specific viruses (ISVs) that do not directly infect vertebrates. Mounting evidence indicates that ISVs interact with arboviruses and may affect mosquito vector competence. Here, we review our current knowledge about the virome of vector mosquitoes and discuss the challenges for the field that may lead to novel strategies to prevent outbreaks of arboviruses.},
note = {1879-6265 (Electronic)
1879-6257 (Linking)
Journal Article
Review},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Leite, Thjf; Ferreira, A G A; Imler, J L; Marques, J T
Distinct Roles of Hemocytes at Different Stages of Infection by Dengue and Zika Viruses in Aedes aegypti Mosquitoes Article de journal
Dans: Front Immunol, vol. 12, p. 660873, 2021, ISBN: 34093550, (1664-3224 (Electronic) 1664-3224 (Linking) Journal Article).
@article{nokey,
title = {Distinct Roles of Hemocytes at Different Stages of Infection by Dengue and Zika Viruses in Aedes aegypti Mosquitoes},
author = {Thjf Leite and A G A Ferreira and J L Imler and J T Marques},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=34093550},
doi = {10.3389/fimmu.2021.660873},
isbn = {34093550},
year = {2021},
date = {2021-01-01},
journal = {Front Immunol},
volume = {12},
pages = {660873},
abstract = {Aedes aegypti mosquitoes are vectors for arboviruses of medical importance such as dengue (DENV) and Zika (ZIKV) viruses. Different innate immune pathways contribute to the control of arboviruses in the mosquito vector including RNA interference, Toll and Jak-STAT pathways. However, the role of cellular responses mediated by circulating macrophage-like cells known as hemocytes remains unclear. Here we show that hemocytes are recruited to the midgut of Ae. aegypti mosquitoes in response to DENV or ZIKV. Blockade of the phagocytic function of hemocytes using latex beads induced increased accumulation of hemocytes in the midgut and a reduction in virus infection levels in this organ. In contrast, inhibition of phagocytosis by hemocytes led to increased systemic dissemination and replication of DENV and ZIKV. Hence, our work reveals a dual role for hemocytes in Ae. aegypti mosquitoes, whereby phagocytosis is not required to control viral infection in the midgut but is essential to restrict systemic dissemination. Further understanding of the mechanism behind this duality could help the design of vector-based strategies to prevent transmission of arboviruses.},
note = {1664-3224 (Electronic)
1664-3224 (Linking)
Journal Article},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2020
Aguiar, Ergr; Almeida, J P P; Queiroz, L R; Oliveira, L S; Olmo, R P; Faria, Ijds; Imler, J L; Gruber, A; Matthews, B J; Marques, J T
A single unidirectional piRNA cluster similar to the flamenco locus is the major source of EVE-derived transcription and small RNAs in Aedes aegypti mosquitoes Article de journal
Dans: Rna, vol. 26, no. 5, p. 581-594, 2020, ISBN: 31996404, (1469-9001 (Electronic) 1355-8382 (Linking) Journal Article Research Support, Non-U.S. Gov't).
@article{nokey,
title = {A single unidirectional piRNA cluster similar to the flamenco locus is the major source of EVE-derived transcription and small RNAs in Aedes aegypti mosquitoes},
author = {Ergr Aguiar and J P P Almeida and L R Queiroz and L S Oliveira and R P Olmo and Ijds Faria and J L Imler and A Gruber and B J Matthews and J T Marques},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=31996404},
doi = {10.1261/rna.073965.119},
isbn = {31996404},
year = {2020},
date = {2020-01-01},
journal = {Rna},
volume = {26},
number = {5},
pages = {581-594},
abstract = {Endogenous viral elements (EVEs) are found in many eukaryotic genomes. Despite considerable knowledge about genomic elements such as transposons (TEs) and retroviruses, we still lack information about nonretroviral EVEs. Aedes aegypti mosquitoes have a highly repetitive genome that is covered with EVEs. Here, we identified 129 nonretroviral EVEs in the AaegL5 version of the A. aegypti genome. These EVEs were significantly associated with TEs and preferentially located in repeat-rich clusters within intergenic regions. Genome-wide transcriptome analysis showed that most EVEs generated transcripts although only around 1.4% were sense RNAs. The majority of EVE transcription was antisense and correlated with the generation of EVE-derived small RNAs. A single genomic cluster of EVEs located in a 143 kb repetitive region in chromosome 2 contributed with 42% of antisense transcription and 45% of small RNAs derived from viral elements. This region was enriched for TE-EVE hybrids organized in the same coding strand. These generated a single long antisense transcript that correlated with the generation of phased primary PIWI-interacting RNAs (piRNAs). The putative promoter of this region had a conserved binding site for the transcription factor Cubitus interruptus, a key regulator of the flamenco locus in Drosophila melanogaster Here, we have identified a single unidirectional piRNA cluster in the A. aegypti genome that is the major source of EVE transcription fueling the generation of antisense small RNAs in mosquitoes. We propose that this region is a flamenco-like locus in A. aegypti due to its relatedness to the major unidirectional piRNA cluster in Drosophila melanogaster.},
note = {1469-9001 (Electronic)
1355-8382 (Linking)
Journal Article
Research Support, Non-U.S. Gov't},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Cai, H; Holleufer, A; Simonsen, B; Schneider, J; Lemoine, A; Gad, H H; Huang, J; Huang, J; Chen, D; Peng, T; Marques, J T; Hartmann, R; Martins, N E; Imler, J L
2'3'-cGAMP triggers a STING- and NF-kappaB-dependent broad antiviral response in Drosophila Article de journal
Dans: Sci Signal, vol. 13, no. 660, 2020, ISBN: 33262294, (1937-9145 (Electronic) 1945-0877 (Linking) Journal Article Research Support, Non-U.S. Gov't).
@article{nokey,
title = {2'3'-cGAMP triggers a STING- and NF-kappaB-dependent broad antiviral response in Drosophila},
author = {H Cai and A Holleufer and B Simonsen and J Schneider and A Lemoine and H H Gad and J Huang and J Huang and D Chen and T Peng and J T Marques and R Hartmann and N E Martins and J L Imler},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=33262294},
doi = {10.1126/scisignal.abc4537},
isbn = {33262294},
year = {2020},
date = {2020-01-01},
journal = {Sci Signal},
volume = {13},
number = {660},
abstract = {We previously reported that an ortholog of STING regulates infection by picorna-like viruses in Drosophila In mammals, STING is activated by the cyclic dinucleotide 2'3'-cGAMP produced by cGAS, which acts as a receptor for cytosolic DNA. Here, we showed that injection of flies with 2'3'-cGAMP induced the expression of dSTING-regulated genes. Coinjection of 2'3'-cGAMP with a panel of RNA or DNA viruses resulted in substantially reduced viral replication. This 2'3'-cGAMP-mediated protection was still observed in flies with mutations in Atg7 and AGO2, genes that encode key components of the autophagy and small interfering RNA pathways, respectively. By contrast, this protection was abrogated in flies with mutations in the gene encoding the NF-kappaB transcription factor Relish. Transcriptomic analysis of 2'3'-cGAMP-injected flies revealed a complex response pattern in which genes were rapidly induced, induced after a delay, or induced in a sustained manner. Our results reveal that dSTING regulates an NF-kappaB-dependent antiviral program that predates the emergence of interferons in vertebrates.},
note = {1937-9145 (Electronic)
1945-0877 (Linking)
Journal Article
Research Support, Non-U.S. Gov't},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Donelick, H M; Talide, L; Bellet, M; Aruscavage, P J; Lauret, E; Aguiar, Ergr; Marques, J T; Meignin, C; Bass, B L
In vitro studies provide insight into effects of Dicer-2 helicase mutations in Drosophila melanogaster Article de journal
Dans: Rna, vol. 26, no. 12, p. 1847-1861, 2020, ISBN: 32843367, (1469-9001 (Electronic) 1355-8382 (Linking) Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't).
@article{nokey,
title = {In vitro studies provide insight into effects of Dicer-2 helicase mutations in Drosophila melanogaster},
author = {H M Donelick and L Talide and M Bellet and P J Aruscavage and E Lauret and Ergr Aguiar and J T Marques and C Meignin and B L Bass},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=32843367},
doi = {10.1261/rna.077289.120},
isbn = {32843367},
year = {2020},
date = {2020-01-01},
journal = {Rna},
volume = {26},
number = {12},
pages = {1847-1861},
abstract = {In vitro, Drosophila melanogaster Dicer-2 (Dcr-2) uses its helicase domain to initiate processing of dsRNA with blunt (BLT) termini, and its Platform*PAZ domain to initiate processing of dsRNA with 3' overhangs (ovrs). To understand the relationship of these in vitro observations to roles of Dcr-2 in vivo, we compared in vitro effects of two helicase mutations to their impact on production of endogenous and viral siRNAs in flies. Consistent with the importance of the helicase domain in processing BLT dsRNA, both point mutations eliminated processing of BLT, but not 3'ovr, dsRNA in vitro. However, the mutations had different effects in vivo. A point mutation in the Walker A motif of the Hel1 subdomain, G31R, largely eliminated production of siRNAs in vivo, while F225G, located in the Hel2 subdomain, showed reduced levels of endogenous siRNAs, but did not significantly affect virus-derived siRNAs. In vitro assays monitoring dsRNA cleavage, dsRNA binding, ATP hydrolysis, and binding of the accessory factor Loquacious-PD provided insight into the different effects of the mutations on processing of different sources of dsRNA in flies. Our in vitro studies suggest effects of the mutations in vivo relate to their effects on ATPase activity, dsRNA binding, and interactions with Loquacious-PD. Our studies emphasize the importance of future studies to characterize dsRNA termini as they exist in Drosophila and other animals.},
note = {1469-9001 (Electronic)
1355-8382 (Linking)
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't},
keywords = {},
pubstate = {published},
tppubtype = {article}
}