Carine Meignin, PhD Group leader at UPR 9022 – M3i CNRS
Contact
Carine Meignin
Phone
E-Mail
Website
UPR 9022 – M3I CNRS
Institut de Biologie Moléculaire et Cellulaire du CNRS
2 allée Konrad Roentgen
67084 Strasbourg Cedex
France
Research topics
- Identification of new RNA sensors
- Localisation and dynamics of antiviral pathway during infection.
- Sensing and processing of viral RNA by Dicer-2 in vivo
Role in NetRNA
The group of Carine Meignin will contribute expertise in antiviral immunity to the network. Tools are generated to investigate the cell biology of antiviral pathway using live imaging.
Expertise
Drosophila genetics
Live imaging
Working Group
Publications
2020
Donelick, H M; Talide, L; Bellet, M; Aruscavage, P J; Lauret, E; Aguiar, Ergr; Marques, J T; Meignin, C; Bass, B L
In vitro studies provide insight into effects of Dicer-2 helicase mutations in Drosophila melanogaster Article de journal
Dans: Rna, vol. 26, no. 12, p. 1847-1861, 2020, ISBN: 32843367, (1469-9001 (Electronic) 1355-8382 (Linking) Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't).
@article{nokey,
title = {In vitro studies provide insight into effects of Dicer-2 helicase mutations in Drosophila melanogaster},
author = {H M Donelick and L Talide and M Bellet and P J Aruscavage and E Lauret and Ergr Aguiar and J T Marques and C Meignin and B L Bass},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=32843367},
doi = {10.1261/rna.077289.120},
isbn = {32843367},
year = {2020},
date = {2020-01-01},
journal = {Rna},
volume = {26},
number = {12},
pages = {1847-1861},
abstract = {In vitro, Drosophila melanogaster Dicer-2 (Dcr-2) uses its helicase domain to initiate processing of dsRNA with blunt (BLT) termini, and its Platform*PAZ domain to initiate processing of dsRNA with 3' overhangs (ovrs). To understand the relationship of these in vitro observations to roles of Dcr-2 in vivo, we compared in vitro effects of two helicase mutations to their impact on production of endogenous and viral siRNAs in flies. Consistent with the importance of the helicase domain in processing BLT dsRNA, both point mutations eliminated processing of BLT, but not 3'ovr, dsRNA in vitro. However, the mutations had different effects in vivo. A point mutation in the Walker A motif of the Hel1 subdomain, G31R, largely eliminated production of siRNAs in vivo, while F225G, located in the Hel2 subdomain, showed reduced levels of endogenous siRNAs, but did not significantly affect virus-derived siRNAs. In vitro assays monitoring dsRNA cleavage, dsRNA binding, ATP hydrolysis, and binding of the accessory factor Loquacious-PD provided insight into the different effects of the mutations on processing of different sources of dsRNA in flies. Our in vitro studies suggest effects of the mutations in vivo relate to their effects on ATPase activity, dsRNA binding, and interactions with Loquacious-PD. Our studies emphasize the importance of future studies to characterize dsRNA termini as they exist in Drosophila and other animals.},
note = {1469-9001 (Electronic)
1355-8382 (Linking)
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Rousseau, C; Meignin, C
Viral sensing by RNA helicases Article de journal
Dans: Virologie (Montrouge), vol. 24, no. 6, p. 36-52, 2020, ISBN: 33441288, (1267-8694 (Print) 1267-8694 (Linking) Journal Article Research Support, Non-U.S. Gov't Review).
@article{nokey,
title = {Viral sensing by RNA helicases},
author = {C Rousseau and C Meignin},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=33441288},
doi = {10.1684/vir.2020.0872},
isbn = {33441288},
year = {2020},
date = {2020-01-01},
journal = {Virologie (Montrouge)},
volume = {24},
number = {6},
pages = {36-52},
abstract = {A key aspect of antiviral immunity is the distinction between "self" and "non-self" components. This distinction can be established through the detection of double-stranded RNA (dsRNA), a common sign of viral infection, by cytosolic RNA helicases. Depending on the organism, two major antiviral pathways can be induced by dsRNA helicases: RNA interference (RNAi) and interferon (IFN) signaling. In the RNAi pathway, dsRNAs are recognized by a Dicer protein, and are then used for the sequence-dependent recognition and subsequent degradation of the complementary viral RNAs. In the IFN signaling pathway, dsRNAs are recognized by a RIG-like receptor (RLR), which induces a signaling cascade in order to induce the expression of IFNs, cytokines and chemokines. In this review, we discuss the RNA features that can be used by the cell to detect a viral infection, the two aforementioned types of helicase-mediated sensing, as well as some viral escape mechanisms developed to avoid recognition.},
note = {1267-8694 (Print)
1267-8694 (Linking)
Journal Article
Research Support, Non-U.S. Gov't
Review},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Talide, L; Imler, J L; Meignin, C
Sensing Viral Infections in Insects: A Dearth of Pathway Receptors Article de journal
Dans: Curr Issues Mol Biol, vol. 34, p. 31-60, 2020, ISBN: 31167955, (1467-3045 (Electronic) 1467-3037 (Linking) Journal Article).
@article{nokey,
title = {Sensing Viral Infections in Insects: A Dearth of Pathway Receptors},
author = {L Talide and J L Imler and C Meignin},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=31167955},
doi = {10.21775/cimb.034.031},
isbn = {31167955},
year = {2020},
date = {2020-01-01},
journal = {Curr Issues Mol Biol},
volume = {34},
pages = {31-60},
abstract = {Insects, the most diverse group of animals, can be infected by an extraordinary diversity of viruses. Among them, arthropod-borne viruses can be transmitted to humans, while bee and silkworm viruses cause important economic losses. Like all invertebrates, insects rely solely on innate immunity to counter viral infections. Protein-based mechanisms, involving restriction factors and evolutionarily conserved signaling pathways regulating transcription factors of the NF-kB and STAT families, participate in the control of viral infections in insects. In addition, RNA-based responses play a major role in the silencing of viral RNAs. We review here our current state of knowledge on insect antiviral defense mechanisms, which include conserved as well as adaptive, insect-specific strategies. Identification of the innate immunity receptors that sense viral infection in insects remains a major challenge for the field.},
note = {1467-3045 (Electronic)
1467-3037 (Linking)
Journal Article},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Talide, L; Imler, J L; Meignin, C
Sensing Viral Infections in Insects: A Dearth of Pathway Receptors Article de journal
Dans: Curr Issues Mol Biol, vol. 34, p. 31-60, 2020, ISBN: 31167955, (1467-3045 (Electronic) 1467-3037 (Linking) Journal Article).
@article{nokey,
title = {Sensing Viral Infections in Insects: A Dearth of Pathway Receptors},
author = {L Talide and J L Imler and C Meignin},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=31167955},
doi = {10.21775/cimb.034.031},
isbn = {31167955},
year = {2020},
date = {2020-01-01},
journal = {Curr Issues Mol Biol},
volume = {34},
pages = {31-60},
abstract = {Insects, the most diverse group of animals, can be infected by an extraordinary diversity of viruses. Among them, arthropod-borne viruses can be transmitted to humans, while bee and silkworm viruses cause important economic losses. Like all invertebrates, insects rely solely on innate immunity to counter viral infections. Protein-based mechanisms, involving restriction factors and evolutionarily conserved signaling pathways regulating transcription factors of the NF-kB and STAT families, participate in the control of viral infections in insects. In addition, RNA-based responses play a major role in the silencing of viral RNAs. We review here our current state of knowledge on insect antiviral defense mechanisms, which include conserved as well as adaptive, insect-specific strategies. Identification of the innate immunity receptors that sense viral infection in insects remains a major challenge for the field.},
note = {1467-3045 (Electronic)
1467-3037 (Linking)
Journal Article},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2019
Martins, N; Lemoine, A; Santiago, E; Paro, S; Imler, J L; Meignin, C
A Transgenic Flock House Virus Replicon Reveals an RNAi Independent Antiviral Mechanism Acting in Drosophila Follicular Somatic Cells Article de journal
Dans: G3 (Bethesda), vol. 9, no. 2, p. 403-412, 2019, ISBN: 30530643, (2160-1836 (Electronic) 2160-1836 (Linking) Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't).
@article{nokey,
title = {A Transgenic Flock House Virus Replicon Reveals an RNAi Independent Antiviral Mechanism Acting in Drosophila Follicular Somatic Cells},
author = {N Martins and A Lemoine and E Santiago and S Paro and J L Imler and C Meignin},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=30530643},
doi = {10.1534/g3.118.200872},
isbn = {30530643},
year = {2019},
date = {2019-01-01},
journal = {G3 (Bethesda)},
volume = {9},
number = {2},
pages = {403-412},
abstract = {The small interfering RNA (siRNA) pathway is the main and best studied invertebrate antiviral response. Other poorly characterized protein based antiviral mechanisms also contribute to the control of viral replication in insects. In addition, it remains unclear whether tissue specific factors contribute to RNA and protein-based antiviral immunity mechanisms. In vivo screens to identify such factors are challenging and time consuming. In addition, the scored phenotype is usually limited to survival and/or viral load. Transgenic viral replicons are valuable tools to overcome these limitations and screen for novel antiviral factors. Here we describe transgenic Drosophila melanogaster lines encoding a Flock House Virus-derived replicon (FHVB2eGFP), expressing GFP as a reporter of viral replication. This replicon is efficiently controlled by the siRNA pathway in most somatic tissues, with GFP fluorescence providing a reliable marker for the activity of antiviral RNAi. Interestingly, in follicular somatic cells (FSC) of ovaries, this replicon is still partially repressed in an siRNA independent manner. We did not detect replicon derived Piwi-interacting RNAs in FSCs and identified 31 differentially expressed genes between restrictive and permissive FSCs. Altogether, our results uncovered a yet unidentified RNAi-independent mechanism controlling FHV replication in FSCs of ovaries and validate the FHVB2eGFP replicon as a tool to screen for novel tissue specific antiviral mechanisms.},
note = {2160-1836 (Electronic)
2160-1836 (Linking)
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't},
keywords = {},
pubstate = {published},
tppubtype = {article}
}